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必威官网手机版>必威体育官网备用亚洲>>Promega试剂>M7745 Pfu DNA Polymerase 500u Promega试剂

M7745 Pfu DNA Polymerase 500u Promega试剂

M7745 Pfu DNA Polymerase 500u Promega试剂
M7745 Pfu DNA Polymerase 500u Promega试剂

M7745 Pfu DNA Polymerase 500u Promega试剂
规格:500u
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产品先容

M7745 Pfu DNA Polymerase 500u Promega试剂

规格:500U

M7745 Pfu DNA Polymerase 500u Promega试剂 产品描述:

其它组分:

 

Pfu DNA Polymerase【子货号:M774B,包装:1 x 500u,,运保温度:-20℃】

Pfu 10X Reaction Buffer w/20mM MgSO4【子货号:M776A,包装:3 x 1.2ml,,运保温度:-20℃】

 

 

描述:

 

 

说明

 

Pfu DNA Polymerase 是一种从强烈炽热球菌(Pyrococcus furiosus ) 中分离得到的热稳定性酶,分子量大约为90kDa。该酶在75℃时可进行DNA 复制,在镁离子存在的条件下可催化核苷酸沿5` → 3` 方向发生聚合反应,形成双链DNA。 Pfu DNA 聚合酶也具有3` → 5` 外切酶( 校正) 活性。当聚合反应发生碱基错配时,聚合酶的校正活性可将错配的碱基切除。因而,推荐Pfu DNA 聚合酶用于高保真的PCR 反应和引物的延伸反应。Pfu DNA 聚合酶产生的PCR产物为平端。

Pfu DNA 聚合酶的10× 反应缓冲液( 含有MgSO4):200mM Tris-HCl (pH 8.8,25℃ ), 100mM KCl, 100mM (NH4)2SO4, 20mM MgSO4, 1.0% Triton® X-100 和1mg/ml 无核酸酶的BSA。

 

特点

 

. 高保真:在所有热稳定性聚合酶中Pfu DNA 聚合酶的错误几率zui低。

. 完备::提供含有20mM MgSO4 的10× 反应缓冲液。

. 品质保证:Promega 企业的PCR 系统,酶和试剂被证明在PCR中能得到可靠高效的结果。

 

 

 

原厂资料:

 

 

Pfu DNA Polymerase is a thermostable enzyme of approximay 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium. Pfu DNA Polymerase also possesses 3´→5´ exonuclease (proofreading) activity. Base misinsertions that may occur during polymerization are rapidly excised by the proofreading activity of the polymerase. Consequently, PfuDNA Polymerase is recommended for use in PCR and primer extension reactions that require high-fidelity synthesis. Pfu DNA Polymerase-generated PCR fragments are blunt-ended.

Pfu DNA Polymerase 10X Reaction Buffer with MgSO4: 200mM Tris-HCl (pH 8.8 at 25°C), 100mM KCl, 100mM (NH4)2SO4, 20mM MgSO4, 1.0% Triton® X-100 and 1mg/ml nuclease-free BSA.

Features - Benefits

  • High Fidelity: Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase.
  • Complete: Provided with 10X buffer containing 20mM MgSO4.
  • Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not compley satisfied with any Promega PCR product, we will send a replacement or refund your account.

Guarantee

PCR Satisfaction Guarantee

PCR Satisfaction Guarantee

Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not compley satisfied with any Promega PCR product we will send a replacement or refund your account.That's Our PCR Guarantee!

Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests preformed.

Applications

Pfu DNA Polymerase is recommended for use in PCR, primer extension reactions and other applications that demand high fidelity.

For more information, see the Protocols & Applications Guide.

References

  1. Fiala, G. and Stetter, K.O. (1986) Arch. Microbiol. 145, 56.
  2. Lundberg, K.S. et al. (1991) Gene 108, 1–6.
  3. Flaman, J.M. et al. (1994) Nucl. Acids Res. 22, 3259–60.
  4. Cline, J. et al. (1996) Nucl. Acids Res. 24, 3546–51.
  5. Andre, P. et al. (1997) Genome Res. 7, 843–52.

 

 

相关产品列表:

Promega 系列
序号货号  名称 规格
1M1801  T4连接酶         100U 
2M1804      T4连接酶      500U
3M1701      M-MLV              10000U 
4M1705      M-MLV             50000U 
5M5101      AMV                    300U 
6M5108      AMV             1000U  
7M7741      Pfu                      100U
8M7745      Pfu            500U  
9C1101      Olig(dt)15 Primer          20ug 
10M3001      Go-Taq                   100U  
11M3005      Go-Taq                 500U    
12M3008      Go-Taq                2500U     
13U1511      dNTP                   200μl   
14U1515      dNTP                   1000μl  
15A1360      pGEM-T easy              1.2ug   
16A3600      pGEM-T                     1.2ug 
17C1181      Random Primers              20μg  
18N2511      Recombinant RNasin           2500U 
19N2515      Recombinant RNasin      10000U  
20N2611      RNasin Plus                2500U  
21N2615      RNasin Plus              10000U   
22N2111      RNasin                   2500U     
23N2115      RNasin                  10000U   
24M1871      TdT                       300U 
25M1875      TdT                      1500U 
26M2825      CIAP                       1000U  
27M1821      CIAP           1000U            
28M2201      Klenow I      150U        
29M2206      Klenow I        500U           
30M4211      T4 DNA Polymerase  100U      
31M4215      T4 DNA Polymerase   500U          
32M4101      T4 Polynucleotide Kinase 100U          
33M4103      T4 Polynucleotide Kinase  1000U         

产品相关关键字: Promega试剂

返回

 相关同类产品:
Promega普洛麦格C1101 Oligo(dT)15 Primer 20ug 现货  GoTaq DNA Polymerase 100u 5u/μl Promega普洛麦格M3001现货  GoTaq DNA Polymerase 500u 5u/μl Promega普洛麦格M3005现货 
M4103 T4 Polynucleotide Kinase Promega试剂  M4101 T4 Polynucleotide Kinase Promega试剂  M4215 T4 DNA Polymerase Promega试剂 
M4211 T4 DNA Polymerase Promega试剂  M2206 Klenow I Promega试剂  M2201 Klenow I Promega试剂 

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